Four Berkeley Lab projects were awarded $1 million from the DOE’s Technology Commercialization Fund (TCF) to further collaborative research with industry partners on biofuels, bioproducts, energy microgrid technology, and renewable energy technology. Two projects from the Biosciences Area were awarded funding for research to enable rapid strain engineering for the production of biofuels and bioproducts from low-cost gas feedstock, and produce a new aviation biofuel precursor from cellulosic biomass
JGI Helps Enable Chloroplast Mimics
In Science, a team led by researchers at the Max Planck Institute (MPI) for Terrestrial Microbiology and the University of Bordeaux have developed a platform that mimics chloroplast function within a cell-sized system. Photosynthesis harnesses carbon captured from the atmosphere in a process that generates energy. The MPI team led by Tobias Erb, director of the MPI for Terrestrial Microbiology, ultimately aims to develop an artificial photosynthesis pathway that would more efficiently remove carbon from the atmosphere than current natural processes and use it as a sustainable resource for making high-value bioproducts. The work was enabled in part through an approved JGI’s Community Science Program (CSP) Functional Genomic proposal from the Erb lab. Learn more here on the JGI website.
Biosciences Area FY21 LDRD Projects
The projects of 15 Biosciences Area scientists and engineers received funding through the FY21 Laboratory Directed Research and Development (LDRD) program.
Biosciences Area 2020 LDRD Projects
The projects of 14 Biosciences Area scientists and engineers received funding through the FY20 Laboratory Directed Research and Development (LDRD) program. The funded projects span a diverse array of topics and approaches including: developing closed-loop plastics from biogenic feedstocks; reimagining a root system optimized for plant-microbe interactions; and creating computational tools for extracting macromolecular conformational dynamics. Lab-wide, 96 projects were selected from a field of 168 proposals. Biosciences Area efforts account for 18.5 percent of the $23 million allocated.
JGI Helps Optimize Microbial Oil Production
Yarrowia lipolytica is a tantalizing microbial factory, capable of turning abundant biomass carbohydrates—glucose and xylose—into fatty acids that could be used for renewable fuel. In fact, lipids can make up more than 90 percent of the microbe’s dry weight.
In order to better harness these gobs of energy, a team of researchers sought to disrupt every gene in the genome with CRISPR-Cas9 in order to interrogate each one’s function. However, the team first needed to fix a defect in the approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, but these “single guide” RNAs (sgRNAs) can be faulty. Without a good sgRNA, Cas9 is ineffective and leaves the target gene intact: a false negative if the gene is actually essential.
Through the JGI’s Community Science Program, the scientists thus set out to identify which sgRNA sequences were reliable. Click here to read the science highlight on the JGI website.
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