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Microscopic view of fluorescent green and red shapes on a dark background. A flow chart of aminotransferase evolution. An amorphous image of red, white, and blue. Open book with circular representations of microbial genomes above, all against a green background Artistic rendering of biomanufacturing A graphic depicting a flow chart of how CRAGE and CRISPR work together Yeast strains engineered for the biochemical conversion of glucose to value-added products are limited in chemical output due to growth and viability constraints. Cell extracts provide an alternative format for chemical synthesis in the absence of cell growth by isolating the soluble components of lysed cells. By separating the production of enzymes (during growth) and the biochemical production process (in cell-free reactions), this framework enables biosynthesis of diverse chemical products at volumetric productivities greater than the source strains. (Blake Rasor) Gradient of the betaxanthins production in yeast across various different cultures. (Courtesy of Matthew Deaner)

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