In Nature, a team led by researchers at the University of Texas at Austin, the HudsonAlpha Institute for Biotechnology, and DOE Joint Genome Institute has produced a high-quality reference sequence of the complex switchgrass genome. Building off this work, researchers at all four DOE Bioenergy Research Centers—the Great Lakes Bioenergy Research Center, the Center for … Read more »
Technique for Studying Gene Expression Takes Root in Plants
Researchers led by Diane Dickel have successfully adapted an open-source RNA analysis platform to study gene expression in individual plant cells. The method, called Drop-seq, was developed at Harvard Medical School in 2015 and had previously been used only in animal cells. Dickel and her colleagues at the DOE Joint Genome Institute (JGI) teamed up with researchers from UC Davis who had perfected a protoplasting technique for root tissue from Arabidopsis thaliana (mouse-ear cress). After preparing samples of more than 12,000 Arabidopsis root cells, the group was thrilled when the Drop-seq process went smoother than expected. Their results were published in Cell Reports.
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