More than a million species of fungi are estimated to live on this planet, but most of that diversity remains unknown because the fungi have avoided detection and have not been cultured for study in laboratories. A team led by researchers at the Joint Genome Institute has developed a pipeline to generate genomes from single cells of uncultivated fungi. The approach was tested on several uncultivated fungal species representing the earliest evolutionary branches in the fungal genealogy that provide a repertoire of important and valuable gene products.