My lab is interested in determining the structure of large multiprotein complexes. We seek to understand the biochemical function of such systems at the level of molecular biophysics. Our primary research method is high-resolution electron microscopy of single-molecule specimens. In addition, however, we employ electron diffraction of two-dimensional crystals, X-ray diffraction of three dimensional crystals, and spectroscopic techniques when appropriate. A major portion of the effort in my lab continues to be the further development of EM methodology, the goals being (1) to extend such work to the resolution needed for chain tracing and (2) to make high-resolution studies possible for protein complexes with MW as low as 250 kDA.