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A graphic depicting a flow chart of how CRAGE and CRISPR work together Data yielded from RIViT-seq increased the number of sigma factor-gene pairs confirmed in Streptomyces coelicolor from 209 to 399. Here, grey arrows denote previously known regulation and red arrows are regulation identified by RIViT-seq; orange nodes mark sigma factors while gray nodes mark other genes. (Otani, H., Mouncey, N.J. Nat Commun 13, 3502 (2022). https://doi.org/10.1038/s41467-022-31191-w) Artistic rendering of the C1 domain of the PKC enzyme shown bound to its ligand. Jean-Marie Volland at Donner lab.

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